Bath Rugby's Charlie Ewels, Zach Mercer and Elliott Stooke have been retained in the England squad ahead of the first Test of the Quilter Internationals against South Africa at Twickenham Stadium on Saturday, kick-off 15:00.
In addition Courtney Lawes (Northampton Saints) will remain in Portugal to continue his rehabilitation.
The 23-man match day squad will be announced on Thursday before returning to London.
Forwards
Tom Curry (Sale Sharks)
Charlie Ewels (Bath Rugby)
Jamie George (Saracens)
Dylan Hartley (Northampton Saints) co-captain
Alec Hepburn (Exeter Chiefs)
Maro Itoje (Saracens)
George Kruis (Saracens)
Zach Mercer (Bath Rugby)
Ben Moon (Exeter Chiefs)
Brad Shields (Wasps)
Kyle Sinckler (Harlequins)
Elliott Stooke (Bath Rugby)
Harry Williams (Exeter Chiefs)
Mark Wilson (Newcastle Falcons)
Backs
Chris Ashton (Sale Sharks)
Danny Care (Harlequins)
Elliot Daly (Wasps)
Owen Farrell (Saracens) co-captain
George Ford (Leicester Tigers)
Jonny May (Leicester Tigers)
Jack Nowell (Exeter Chiefs)
Henry Slade (Exeter Chiefs)
Ben Te’o (Worcester Warriors)
Manu Tuilagi (Leicester Tigers)
Ben Youngs (Leicester Tigers)