Bath Rugby's Charlie Ewels and Semesa Rokoduguni have been retained in the England squad ahead of Saturday's Old Mutual Wealth Series match against Samoa at Twickenham Stadium, kick-off 15:00.
Nathan Hughes (Wasps) has returned to his club for rehab on his right knee. The number 8 has bone bruising in his knee joint and will require treatment for the next three to four weeks.
Mike Brown (Harlequins), Elliot Daly (Wasps), Jonny May (Leicester Tigers), Semesa Rokoduguni (Bath Rugby)
Danny Care (Harlequins), Owen Farrell (Saracens), George Ford (Leicester Tigers), Piers Francis (Northampton Saints), Alex Lozowski (Saracens), Henry Slade (Exeter Chiefs), Ben Youngs (Leicester Tigers)
Charlie Ewels (Bath Rugby), Nick Isiekwe (Saracens), Maro Itoje (Saracens), Joe Launchbury (Wasps), Courtney Lawes (Northampton Saints), Chris Robshaw (Harlequins), Sam Simmonds (Exeter Chiefs)
Dan Cole (Leicester Tigers), Ellis Genge (Leicester Tigers), Jamie George (Saracens), Dylan Hartley (Northampton Saints), Joe Marler (Harlequins), Mako Vunipola (Saracens), Harry Williams (Exeter Chiefs)
Players released back to their clubs:
Tom Dunn (Bath Rugby)
Nathan Hughes (Wasps)
Jonathan Joseph (Bath Rugby)
George Kruis (Saracens)
Zach Mercer (Bath Rugby)
Marcus Smith (Harlequins)
Denny Solomona (Sale Sharks)
Anthony Watson (Bath Rugby)
Ben Te'o (Worcester Warriors) will remain in camp until Wednesday. Sam Underhill (Bath Rugby) will continue to go through his Graduated Return to Play protocols in camp.